cathelicidin-OA1 peptide

cathelicidin-OA1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-IGRDPTWSHLAASCLKCIFDDLPKTHN-OH

H-Ile-Gly-Arg-Asp-Pro-Thr-Trp-Ser-His-Leu-Ala-Ala-Ser-Cys-Leu-Lys-Cys-Ile-Phe-Asp-Asp-Leu-Pro-Lys-Thr-His-Asn-OH

27

95.99%

C₁₃₄H₂₀₆N₃₈O₃₉S₂

3037.42

Synthetic

Powder

Cathelicidins play pivotal roles in host defense. Cathelicidin-OA1 is a Cathelicidin identified from the skin of an amphibian species, Odorrana andersonii. Cathelicidin-OA1 showed no direct microbe-killing, acute toxicity and hemolytic activity, but did exhibit antioxidant activity. Cathelicidin-OA1 accelerated wound healing against human keratinocytes (HaCaT) and skin fibroblasts (HSF) in both time- and dose-dependent manners. Cathelicidin-OA1 also showed wound-healing promotion in a mouse model with full-thickness skin wounds, accelerating re-epithelialization and granulation tissue formation by enhancing the recruitment of macrophages to the wound site, inducing HaCaT cell proliferation and HSF cell migration.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cao X, Wang Y, Wu C, Li X, Fu Z, Yang M, Bian W, Wang S, Song Y, Tang J, Yang X. Cathelicidin-OA1, a novel antioxidant peptide identified from an amphibian, accelerates skin wound healing. Sci Rep. 2018 Jan 17;8(1):943. doi: 10.1038/s41598-018-19486-9. Erratum in: Sci Rep. 2018 Oct 23;8(1):15906. PMID: 29343843; PMCID: PMC5772731.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.