Casein beta, beta-Casomorphin-5 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Beta-casein is a major milk protein that serves as a precursor for various peptides. Beta-casomorphin-5 (BCM5) is a specific pentapeptide derived from bovine beta-casein, corresponding to the fragment f60-64 of its amino acid sequence. Its structure includes the N-terminal sequence Tyr-Pro-Phe-Pro-Gly.

During simulated gastrointestinal digestion, the release of BCM5 from beta-casein is influenced by genetic variants of the protein. Research indicates that BCM5 is not formed in hydrolysates irrespective of the genetic variant (A1, A2, or B) or the pH conditions during digestion. This contrasts with the related heptapeptide beta-casomorphin-7, which is released from variants A1 and B but not from variant A2, a difference attributed to a proline residue at position 67 in the A2 variant's primary structure.

• Lönnerdal B, Bergström S, Andersson Y, Hjalmarsson K, Sundqvist AK, Hernell O. Cloning and sequencing of a cDNA encoding human milk beta-casein. FEBS Lett. 1990;269(1):153-6.
• Noni ID. Release of β-casomorphins 5 and 7 during simulated gastro-intestinal digestion of bovine β-casein variants and milk-based infant formulas. Food Chem. 2008 Oct 15;110(4):897-903. doi: 10.1016/j.foodchem.2008.02.077. Epub 2008 Mar 5. PMID: 26047276.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.