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Product Name
Mouse Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein
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Description
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
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Protein name
Carbonic anhydrase 9
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Protein short names
RP23-195K8.9; CARBONIC ANHYDRASE 9; MN; CAR9; MN/CA9; CAIX; CA9; CARBONIC ANHYDRASE IX
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Uniprot ID
Q8VHB5
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Gene Name
Car9; mCG_141072
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
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Validations
Mouse Carbonic Anhydrase IX / Car9 Protein (His Tag) SDS-PAGE
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