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Product Name
Mouse Carbonic Anhydrase XIV (His Tag) recombinant protein
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Description
Reversible hydration of carbon dioxide.
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Protein name
Carbonic anhydrase 14
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Protein short names
CARBONIC ANHYDRASE XIV; CAR14; AW536446; CAXIV; CA14; CARBONIC ANHYDRASE 14
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Uniprot ID
Q9WVT6
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Gene Name
Ca14; Car14; Catm
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
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Validations
Mouse Carbonic Anhydrase XIV / Car14 Protein (His Tag) SDS-PAGE
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