Canine TrkB/NTRK2 (His Tag) recombinant protein

Canine TrkB/NTRK2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.

TRK-B; NTRK2; GP145-TRKB/GP95-TRKB; TRKB; GP145-TRKB; AI848316; TKRB; C030027L06RIK

Human Cells

A DNA sequence encoding the canine NTRK (Met1-His430) was expressed with a C-terminal polyhistidine tag.

Canine

The recombinant canine NTRK comprises 410 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized canine NTRK-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse BDNF (cat:500704), The EC₅₀ of biotinylated mouse BDNF (cat:500704) is 0.07-0.17 μg/ml.