Canine NRG1 (Fc Tag) recombinant protein

Canine NRG1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis. 

MSTP131; HRG1; MST131; GGF; NRG1-IT2; SMDF; GGFII; HRG; HGL; NDF; HRGALPHA; HRGA; ARIA; 6030402G23RIK; GGF2; D230005F13RIK

Human Cells

A DNA sequence encoding the canine NRG1 isoform alpha (XP_858147.1) extracellular domain (Ser19-Lys240) was expressed with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine NRG1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 460 amino acids and has a predicted molecular mass of 50.8 kDa. The apparent molecular mass of the protein is approximately 67 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.
The ED₅₀ for this effect is typically 2-12 µg/mL.