Canine NRG1 (EGF Domain, Fc Tag) recombinant protein

Canine NRG1 (EGF Domain, Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis. 

D230005F13RIK; 6030402G23RIK; GGF2; NDF; HGL; ARIA; HRGALPHA; HRGA; GGFII; SMDF; NRG1-IT2; HRG; MSTP131; HRG1; GGF; MST131

Human Cells

A DNA sequence encoding the canine NRG1 isoform alpha (XP_858147.1) EGF-like domain (Ser176-Lys240) was expressed with the Fc region of mouse IgG1 at the N-terminus.

Canine

The recombinant canine NRG1 is a disulfide-linked homodimer. The reduced monomer comprises 301 amino acids and has a predicted molecular mass of 34 kDa. The apparent molecular mass of the protein is approximately 37 and 34 kDa in SDS-PAGE under reducing conditions.

(92.0+7.0) % as determined by SDS-PAGE

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.
The ED₅₀ for this effect is typically 0.1-1 µg/mL.