Canine Ephrin-A5/EFNA5 (Fc Tag) recombinant protein

Canine Ephrin-A5/EFNA5 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

EPLG7; AF1; EFL-5; RAGS; AL-1; EPL7; EFNA5; EFL5; AV158822; LERK7; GLC1M; EPHRIN-A5; LERK-7

Human Cells

A DNA sequence encoding the canine EFNA5 (XP_850582.2) (Met1-Asn203) was expressed with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 424 amino acids and has a predicted molecular mass of 48.2 kDa. The apparent molecular mass of the protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Immobilized human EPHA3-His (Cat:504736) at 10 μg/ml (100 μl/well) can bind canine EFNA5-Fc, The EC₅₀ of canine EFNA5-Fc is 11.5-26.7 ng/ml.