Canine EGFR/HER1/ErbB1 (His Tag) recombinant protein

Canine EGFR/HER1/ErbB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Baculovirus-Insect Cells

A DNA sequence encoding the canine EGFR (XP_533073.3) (Met1-Ser618) was expressed with a C-terminal polyhistidine tag.

Canine

The recombinant canine EGFR comprises 629 amino acids and has a predicted molecular mass of 69.8 kDa. The apparent molecular mass of the protein is approximately 87.2 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized canine EGFR-His at 10 μg/ml (100 μl/well) can bind biotinylated canine His-EGF(Cat:500258), The EC₅₀ of biotinylated canine His-EGF(Cat:500258) is 0.16-0.36μg/ml