Canine CD137 / 4-1BB (His Tag) recombinant protein

Canine CD137 / 4-1BB (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.

LY63; CDW137; CD137; 4-1BB; AA408498; ILA; TNFRSF9; RP23-272N19.6; A930040I11RIK; MGC2172; AI325004

E2R1R9

Human Cells

A DNA sequence encoding the canine TNFRSF9 (XP_850336.1) (Met1-Ser185) was expressed with a C-terminal polyhistidine tag.

Canine

The recombinant canine TNFRSF9 comprises 173 amino acids and has a predicted molecular mass of 18.4 kDa. The apparent molecular mass of the protein is approximately 28-34 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized canine TNFRSF9-His at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9 (Cat:503740), The EC₅₀ of rat Fc-TNFSF9 (Cat:503740) is 0.26-0.62 μg/ml.