Canine CD122 / IL-2RB (Fc Tag) recombinant protein

Canine CD122 / IL-2RB (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

CD122; RP5-1170K4.6; P70-75; IL15RB; IL-2RBETA; IL-2RB; IL-15RBETA; IL2RB; P70; IL15RBETA; IL-2/15RBETA

Human Cells

A DNA sequence encoding the extracellular domain of the canine IL2RB (XP_003431552.1) (Met1-Pro244) was expressed with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine IL2RB/Fc is a disulfide-linked homodimer. The reduced monomer comprises 461 amino acids and has a predicted molecular mass of 52.3 kDa. The apparent molecular mass of the protein is approximately 67 kDa in SDS-PAGE under reducing conditions.

> 80 % as determined by SDS-PAGE