Canine Carbonic Anhydrase IX/CA9 (Fc Tag) recombinant protein

Canine Carbonic Anhydrase IX/CA9 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

MN; CARBONIC ANHYDRASE 9; RP23-195K8.9; CAIX; MN/CA9; CAR9; CARBONIC ANHYDRASE IX; CA9

Human Cells

A DNA sequence encoding the canine CA9 (Met1-Leu410) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine CA9/Fc is a disulfide-linked homodimer. The reduced monomer comprises 614 amino acids and has a predicted molecular mass of 67.6 kDa. The apparent molecular mass of the protein is approximately 79 kDa in SDS-PAGE under reducing conditions.

> 99 % as determined by SDS-PAGE

Measured by its esterase activity.
The specific activity is > 10 pmoles/min/μg.