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Product Name
C3 / C3b / C3c antibody
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Description
C3 / C3b / C3c Rabbit Polyclonal antibody. Positive IP detected in human plasma tissue. Positive WB detected in human blood tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 115 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
ARMD9 antibody; ASP antibody; C3b antibody; C3c antibody; Complement C3 antibody; complement component 3 antibody; CPAMD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of C3 / C3b / C3c recombinant protein (Accession Number: NM_000064). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:50-1:500
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Validations
human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:108718(C3 antibody) at dilution of 1:1000
IP Result of anti-C3 (IP:Catalog No:108718, 4ug; Detection:Catalog No:108718 1:1000) with human plasma tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:108718(C3 Antibody) at dilution of 1:100 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:108718(C3 Antibody) at dilution of 1:100 (under 40x lens).
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Background
The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.
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