C-II-a / ApoC-II-a peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

C-II-a, also known as ApoC-II-a, is a novel bihelical amphipathic peptide designed to mimic the properties of apolipoprotein C-II. It consists of two distinct helical domains: an N-terminal 18A amphipathic helix that confers high affinity for lipid binding to lipoproteins, and a C-terminal helix derived from the last helix of apolipoprotein C-II, which contains the critical motif for activating lipoprotein lipase (LPL). This structural design enables the peptide to effectively associate with lipoprotein surfaces and stimulate LPL-mediated lipolysis of triglycerides.

In vitro characterization demonstrates that the C-II-a peptide is a potent activator of LPL, exhibiting efficacy comparable to the full-length apolipoprotein C-II protein in promoting lipolysis using both artificial lipid emulsions and native lipoprotein substrates. The peptide also facilitates cholesterol efflux via the ATP-binding cassette transporter ABCA1. Biophysical analyses, including structural modeling and circular dichroism spectroscopy, confirm its amphipathic helical conformation and interaction with lipid surfaces.

• Reimund M, Wolska A, Risti R, Wilson S, Sviridov D, Remaley AT, Lookene A. Apolipoprotein C-II mimetic peptide is an efficient activator of lipoprotein lipase in human plasma as studied by a calorimetric approach. Biochem Biophys Res Commun. 2019 Oct 29;519(1):67-72. doi: 10.1016/j.bbrc.2019.08.130. Epub 2019 Aug 30. PMID: 31477272.
• Amar MJ, Sakurai T, Sakurai-Ikuta A, Sviridov D, Freeman L, Ahsan L, Remaley AT. A novel apolipoprotein C-II mimetic peptide that activates lipoprotein lipase and decreases serum triglycerides in apolipoprotein E-knockout mice. J Pharmacol Exp Ther. 2015 Feb;352(2):227-35. doi: 10.1124/jpet.114.220418. Epub 2014 Nov 13. PMID: 25395590; PMCID: PMC4293430.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.