C-GLUT4 peptide

C-GLUT4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KPSTELEYLGPDEND-OH

H-Lys-Pro-Ser-Thr-Glu-Leu-Glu-Tyr-Leu-Gly-Pro-Asp-Glu-Asn-Asp-OH

15

95.24%

C₇₃H₁₁₁N₁₇O₃₀

1706.75

Synthetic

Powder

It is a synthetic peptide of the C-terminal domain of rat GLUT4, a glucose transporter isoform. The peptide can be used to produce its corresponding antibody aGLUT4-C, which can specifically recognizes rat GLUT4 and does not react with other isoforms such as GLUT1 or GLUT2. The antibody was prepared using a short peptide affinity column and was used in immunoblotting and immunocytochemical studies to detect and localize GLUT4 protein. It was used to stain CHO-GLUT4 cells, where it showed specific staining in a specific cytoplasmic region. The subcellular localization of exogenous GLUT4 was not altered by insulin treatment. The amount of GLUT4 protein was estimated using a peptide-based radioimmunoassay system specific for GLUT4. The expression of exogenous rat GLUT4 in 3T3-L1 adipocytes did not respond to insulin, possibly due to the high expression of endogenous mouse GLUT4. Additional cellular factors, such as GLUT4-specific vesicles, may be required for a large insulin response in fibroblasts.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Shibasaki Y, Asano T, Lin JL, Tsukuda K, Katagiri H, Ishihara H, Yazaki Y, Oka Y. Two glucose transporter isoforms are sorted differentially and are expressed in distinct cellular compartments. Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):829-34. doi: 10.1042/bj2810829. PMID: 1536658; PMCID: PMC1130764.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.