BRP/BRINP2-related peptide

BRP/BRINP2-related peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-THRILRRLFNLC-NH2

H-Thr-His-Arg-Ile-Leu-Arg-Arg-Leu-Phe-Asn-Leu-Cys-NH2

12

95.39%

C₆₈H₁₁₇N₂₅O₁₄S

1540.87

Synthetic

Powder

BRP (THRILRRLFNLC) is a 12-mer amidated peptide (BRINP2-related peptide) discovered via Peptide Predictor, a computational–functional screen of PCSK prohormone-processing sites. It is cleaved from the 78-kDa secreted protein BRINP2 by proconvertases (PCSK1 in part) at flanking KK/KR sites; C-terminal amidation is required for bioactivity. AlphaFold predicts an α-helical or linear conformation. BRINP2 is brain-predominant in mice, humans and pigs; endogenous BRP is detected in human CSF (700 pM–3 nM) and mouse brain (16–140 pM). Pharmacologically, BRP suppresses food intake and reverses obesity in lean, diet-induced-obese, male and female mice, and reduces feeding in minipigs—without altering blood glucose, insulin secretion, or inducing nausea/aversion. Its action is independent of GLP1R, leptin and MC4R, instead activating central CREB–FOS signalling in hypothalamic nuclei (DMH, preoptic, tuberal, arcuate).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Coassolo L, B Danneskiold-Samsøe N, Nguyen Q, Wiggenhorn A, Zhao M, Wang DC, Toomer D, Lone J, Wei Y, Patel A, Liparulo I, Kavi D, Wat LW, Reghupaty SC, Kim JJ, Asemi T, Bielczyk-Maczynska E, Li VL, Moya-Garzon MD, Krentz NAJ, Stahl A, Chou DH, Luo L, Svensson KJ. Prohormone cleavage prediction uncovers a non-incretin anti-obesity peptide. Nature. 2025 May;641(8061):192-201. doi: 10.1038/s41586-025-08683-y. Epub 2025 Mar 5. PMID: 40044869; PMCID: PMC12043402.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.