Brevinin-2LF1 peptide

Brevinin-2LF1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GFMDTAKNVAKNVAKNVAVTLLDKLRCKVTGGC-OH

H-Gly-Phe-Met-Asp-Thr-Ala-Lys-Asn-Val-Ala-Lys-Asn-Val-Ala-Lys-Asn-Val-Ala-Val-Thr-Leu-Leu-Asp-Lys-Leu-Arg-Cys-Lys-Val-Thr-Gly-Gly-Cys-OH (disulfide bond: Cys27-Cys33)

33

97%

C₁₄₉H₂₅₆N₄₄O₄₄S₃

3464.15

Synthetic

Powder

Brevinin-2LF1 is a cationic antimicrobial peptide identified from the skin secretions of Amolops lifanensis, an East Asian frog species. This peptide contains 33 amino acid residues with a net charge of +4 at pH 7 and a theoretical pI of 10.70. Its observed molecular mass is 3464.08 Da, consistent with the calculated mass of 3464.14 Da. The peptide features two cysteine residues capable of forming a single intramolecular disulfide bond, a structural characteristic common to many amphibian antimicrobial peptides. Brevinin-2LF1 exhibits antimicrobial activity against a range of microorganisms. It demonstrates high potency against the Gram-negative bacterium Psychrobacter faecalis X29, with a minimum inhibitory concentration (MIC) of 3.1 mM, and also shows activity against Gram-positive bacteria such as Staphylococcus aureus and Nocardia asteroides. The peptide shares a precursor structure similar to other antimicrobial and antioxidant peptides from ranid frogs, consisting of a signal peptide and an N-terminal acidic spacer domain terminated by a dibasic processing site.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Guo C, Hu Y, Li J, et al. Identification of multiple peptides with antioxidant and antimicrobial activities from skin and its secretions of Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus. Biochimie. 2014;105:192-201.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.