Bradykinin-potentiating peptide Phypo Xa

Bradykinin-potentiating peptide Phypo Xa

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Pyroglutamic acid Pyr-FRPSYQIPP-OH

Pyroglutamic acid Pyr-Phe-Arg-Pro-Ser-Tyr-Gln-Ile-Pro-Pro-OH

10

95.44%

C₅₈H₈₂N₁₄O₁₅

1215.35

Synthetic

Powder

Bradykinin-potentiating peptide Phypo Xa is a type of Bradykinin potentiating peptides (BPPs) isolated from the skin secretion of the Brazilian tree-frog Phyllomedusa hypochondrialis, and is the first canonical BPP to be found not only in the frog skin but also in any other natural souce other than the snake venoms.
Bradykinin-potentiating peptide Phypo Xa can potentiate bradykinin activities in vivo and in vitro, as well as efficiently and competitively inhibit ACE. Besides, it presents a classical motif and can be recognized by its typical pyroglutamyl(Pyr)/proline-rich sequences invariably presenting a proline residue at the C-terminus as other BPPs.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conceição K, Konno K, De melo RL, et al. Isolation and characterization of a novel bradykinin potentiating peptide (BPP) from the skin secretion of Phyllomedusa hypochondrialis. Peptides. 2007;28(3):515-23.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.