Biotin-DFYIGSRGD peptide
Not For Human Use, Lab Use Only.
Cat.#: 319264
Special Price 102.3 USD
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Product Name
Biotin-DFYIGSRGD peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Biotin-fYIGSRGD-OH, f=D-phenylalanine
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Sequence
Biotin-D-Phe-Tyr-Ile-Gly-Ser-Arg-Gly-Asp-OH
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Length (aa)
8
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Peptide Purity (HPLC)
95.4%
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Molecular Formula
C51H73N13O15S
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Molecular Weight
1140.26
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Source
Synthetic
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Form
Powder
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Description
Biotin-FYIGSRGD (F=D-phenylalanine) could self-assemble into supramolecular hydrogels (termed SupraGel) for 3D cell culture. The introduction of one D-amino acid in the peptide may decrease cell-matrix interactions, thus facilitating spontaneous cell spheroid formation. The pentapeptide YIGSR is from the functional motif (929–933 sequence of the β1 chain) of laminin, a major glycoprotein located in the basement membrane. It can directly bind to the 67 kDa laminin receptor, a non-integrin cell surface receptor, and regulate cell adhesion, growth, differentiation, and migration. The tripeptide arginine-glycine-aspartate (RGD) is a functional motif that binds to the cell surface receptor integrin and regulates cell behavior.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Ai, S., Li, H., Zheng, H. et al. A SupraGel for efficient production of cell spheroids. Sci. China Mater. 65, 1655-1661 (2022). https://doi.org/10.1007/s40843-021-1951-x
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"