Biotin-^DFYIGSRGD peptide

Biotin-^DFYIGSRGD peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Biotin-fYIGSRGD-OH, f=D-phenylalanine

Biotin-D-Phe-Tyr-Ile-Gly-Ser-Arg-Gly-Asp-OH

8

96.93%

C₅₁H₇₃N₁₃O₁₅S

1140.27

Synthetic

Powder

Biotin-FYIGSRGD (F=D-phenylalanine) could self-assemble into supramolecular hydrogels (termed SupraGel) for 3D cell culture. The introduction of one D-amino acid in the peptide may decrease cell-matrix interactions, thus facilitating spontaneous cell spheroid formation. The pentapeptide YIGSR is from the functional motif (929–933 sequence of the β1 chain) of laminin, a major glycoprotein located in the basement membrane. It can directly bind to the 67 kDa laminin receptor, a non-integrin cell surface receptor, and regulate cell adhesion, growth, differentiation, and migration. The tripeptide arginine-glycine-aspartate (RGD) is a functional motif that binds to the cell surface receptor integrin and regulates cell behavior.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ai, S., Li, H., Zheng, H. et al. A SupraGel for efficient production of cell spheroids. Sci. China Mater. 65, 1655-1661 (2022). https://doi.org/10.1007/s40843-021-1951-x

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.