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Product Name
E. coli BirA / Bifunctional protein BirA (His & MBP Tag)
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Description
Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
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Protein name
Bifunctional ligase/repressor BirA
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Protein short names
B3973; DHBB; JW3941; BIOR
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Uniprot ID
P06709
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Gene Name
birA; bioR; dhbB; b3973; JW3941
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the E.coil BirA (P06709) (Met1–Lys321) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
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Protein Species
E. coli
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Molecular weight
The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
E. coil Bifunctional protein BirA (His & MBP Tag) SDS-PAGE
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