Human BIRC7/Livin (His Tag) recombinant protein

Human BIRC7/Livin (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.

Baculoviral IAP repeat-containing protein 7

RNF50; ML-IAP; E130019N06; KIAP; LIVIN; MLIAP

Q96CA5

BIRC7; KIAP; LIVIN; MLIAP; RNF50; UNQ5800/PRO19607/PRO21344

E. coli

A DNA sequence encoding the human BIRC7 (AAH14475.1) (Met1-Ser298) was expressed with a polyhistide tag at the C-terminus.

Human

The recombinant human BIRC7 consists of 304 amino acids and predicts a molecular mass of 33.6 kDa. It migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its ability to reverse the inhibition of DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The IC50 for this effect is typically 0.6-3.0 μM.