BacFL31 peptide

BacFL31 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GLEESPGHPGQPGPPGPPGAPGP

Gly-Leu-Glu-Glu-Ser-Pro-Gly-His-Pro-Gly-Gln-Pro-Gly-Pro-Pro-Gly-Pro-Pro-Gly-Ala-Pro-Gly-Pro

23

95.6%

Synthetic

Powder

Warning: the reported sequence is incomplete. BacFL31 was purified to homogeneity by four purification steps (heat treatment 15 min at 90 °C, 60% ammonium sulphate precipitation, gel filtration Sephadex G25, and reverse phase HPLC). It is usual that even in the N-terminal 23 residues sequenced, six prolines at positions 6,9,12,15,18, and 21 are hydroxylated (XXK). Active against L. monocytogenes. It may be developed into a food preservative. BacFL31 was found in Enterococcus faecium FL31.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Chakchouk-mtibaa A, Elleuch L, Smaoui S, et al. An antilisterial bacteriocin BacFL31 produced by Enterococcus faecium FL31 with a novel structure containing hydroxyproline residues. Anaerobe. 2014;27:1-6.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.