BacFL31 peptide

BacFL31 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GLEESPGHPGQPGPPGPPGAPGP

Gly-Leu-Glu-Glu-Ser-Pro-Gly-His-Pro-Gly-Gln-Pro-Gly-Pro-Pro-Gly-Pro-Pro-Gly-Ala-Pro-Gly-Pro

23

95.6%

Synthetic

Powder

Warning: the reported sequence is incomplete. BacFL31 was purified to homogeneity by four purification steps (heat treatment 15 min at 90 °C, 60% ammonium sulphate precipitation, gel filtration Sephadex G25, and reverse phase HPLC). It is usual that even in the N-terminal 23 residues sequenced, six prolines at positions 6,9,12,15,18, and 21 are hydroxylated (XXK). Active against L. monocytogenes. It may be developed into a food preservative. BacFL31 was found in Enterococcus faecium FL31.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Chakchouk-mtibaa A, Elleuch L, Smaoui S, et al. An antilisterial bacteriocin BacFL31 produced by Enterococcus faecium FL31 with a novel structure containing hydroxyproline residues. Anaerobe. 2014;27:1-6.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.