Bac7(1-35) peptide

Bac7(1-35) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRIRPRPPRLPRPRPRPLPFPRPGPRPIPRPLPFP

Arg-Arg-Ile-Arg-Pro-Arg-Pro-Pro-Arg-Leu-Pro-Arg-Pro-Arg-Pro-Arg-Pro-Leu-Pro-Phe-Pro-Arg-Pro-Gly-Pro-Arg-Pro-Ile-Pro-Arg-Pro-Leu-Pro-Phe-Pro

35

98.17%

C₁₉₆H₃₂₂N₆₈O₃₆

4207.06

Synthetic

Powder

Bac7(1-35) is a proline-rich AMP belonging to the cathelicidin family, can reach high concentrations (up to 340 μM) inside the E. coli cytoplasm. The peptide specifically and completely inhibits in vitro translation in the micromolar concentration range. Bac7(1–35) is a fully active fragment of Bac7, which translocates into susceptible bacteria by using a specific transporter, where it then interferes with ribosomes. Bac7(1–35), after crossing the membrane without lysis, acts by targeting ribosomes. Bac7(1–35) CD spectrum in phosphate buffer recalls that of the polyproline II conformation, which is not surprising considering the high percentage of proline residues in its primary structure.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mardirossian M, Grzela R, Giglione C, et al. The host antimicrobial peptide Bac71-35 binds to bacterial ribosomal proteins and inhibits protein synthesis.
• Benincasa, Monica et al. “Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides.” Microorganisms vol. 4,3 26. 10 Aug. 2016, doi:10.3390/microorganisms4030026

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.