Bac7(1-35) peptide

Bac7(1-35) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRIRPRPPRLPRPRPRPLPFPRPGPRPIPRPLPFP

Arg-Arg-Ile-Arg-Pro-Arg-Pro-Pro-Arg-Leu-Pro-Arg-Pro-Arg-Pro-Arg-Pro-Leu-Pro-Phe-Pro-Arg-Pro-Gly-Pro-Arg-Pro-Ile-Pro-Arg-Pro-Leu-Pro-Phe-Pro

35

98.08%

C₁₉₆H₃₂₂N₆₈O₃₆

4207.06

Synthetic

Powder

Bac7(1-35) is a proline-rich AMP belonging to the cathelicidin family, can reach high concentrations (up to 340 μM) inside the E. coli cytoplasm. The peptide specifically and completely inhibits in vitro translation in the micromolar concentration range. Bac7(1–35) is a fully active fragment of Bac7, which translocates into susceptible bacteria by using a specific transporter, where it then interferes with ribosomes. Bac7(1–35), after crossing the membrane without lysis, acts by targeting ribosomes. Bac7(1–35) CD spectrum in phosphate buffer recalls that of the polyproline II conformation, which is not surprising considering the high percentage of proline residues in its primary structure.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mardirossian M, Grzela R, Giglione C, et al. The host antimicrobial peptide Bac71-35 binds to bacterial ribosomal proteins and inhibits protein synthesis.
• Benincasa, Monica et al. “Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides.” Microorganisms vol. 4,3 26. 10 Aug. 2016, doi:10.3390/microorganisms4030026

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.