Bac5 peptide

Bac5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-RFRPPIRRPPIRPPFYPPFRPPIRPPIFPPIRPPFRPPLGPFP-OH

H-Arg-Phe-Arg-Pro-Pro-Ile-Arg-Arg-Pro-Pro-Ile-Arg-Pro-Pro-Phe-Tyr-Pro-Pro-Phe-Arg-Pro-Pro-Ile-Arg-Pro-Pro-Ile-Phe-Pro-Pro-Ile-Arg-Pro-Pro-Phe-Arg-Pro-Pro-Leu-Gly-Pro-Phe-Pro-OH

43

95.77%

C₂₅₅H₃₈₂N₇₀O₄₅

5148.19

Synthetic

Powder

Bac5 is a bovine antimicrobial peptide (AMP) that has been shown to have chemotactic properties and induce inflammatory cytokine expression by innate immune cells. It is capable of killing some species of bacteria through the inhibition of protein synthesis. Bac5 has been investigated for its potential use as a novel vaccine adjuvant. In vitro studies using murine macrophage-like cells have shown that Bac5 can trigger the release of nitric oxide and upregulate surface marker expression, including CD86, MHC-I, and MHC-II. When coupled with mycobacterial antigen and Bacillus subtilis spores, Bac5 enhances IFNγ secretion.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Munshi T, Sparrow A, Wren BW, Reljic R, Willcocks SJ. The Antimicrobial Peptide, Bactenecin 5, Supports Cell-Mediated but Not Humoral Immunity in the Context of a Mycobacterial Antigen Vaccine Model. Antibiotics (Basel). 2020 Dec 19;9(12):926. doi: 10.3390/antibiotics9120926. PMID: 33352656; PMCID: PMC7766334.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.