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Product Name
ARPP-19 antibody
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Description
ARPP-19 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in Jurkat cells, A375 cells, A549 cells, mouse brain tissue, rat skeletal muscle tissue. Positive IF detected in A375 cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 19kd
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARPP 16 antibody; ARPP 19 antibody; ARPP16 antibody; ARPP19 antibody; ARPP-19 antibody; FLJ41622 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARPP-19 recombinant protein (Accession Number: NM_006628). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108170(ARPP-19 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108170(ARPP-19 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-ARPP-19 (IP:Catalog No:108170, 3ug; Detection:Catalog No:108170 1:500) with mouse brain tissue lysate 5200ug.
Immunofluorescent analysis of A375 cells using Catalog No:108170(ARPP-19 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ARPP-19, a 19-kDa cAMP-regulated phosphoprotein, plays a role in regulating mitosis. Initiation and maintenance of mitosis require the activation of protein kinase cyclin B–Cdc2 and the inhibition of protein phosphatase 2A (PP2A). When phosphorylated by protein kinase Greatwall (Gwl), ARPP-19 associate with and inhibit PP2A, thus promoting mitotic entry. ARPP-19 may be an important link between nerve growth factor (NGF) signaling and post-transcriptional control of neuronal gene expression such as GAP-43.
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References
- Song H, Pan J, Liu Y. Increased ARPP-19 expression is associated with hepatocellular carcinoma. International journal of molecular sciences. 16(1):178-92. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"