Mouse MANF (His Tag) recombinant protein

Mouse MANF (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF and ARMET, is a secreted protein which belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types.

Arginine-rich, mutated in early stage tumors, isoform CRA_b

AA673178; MGC142150; MGC142148; AA408789; 3230402M22RIK; D17914; ARP; ARMET; D18MGI17; MANF; AA407711

Q3TMX5

Manf; Armet; mCG_19507

Human Cells

A DNA sequence encoding the mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse MANF comprises 169 amino acids and has a calculated molecular mass of 19.6 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE