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Product Name
ARFGAP3 antibody
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Description
ARFGAP3 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in A431 cells, HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells, mouse brain tissue. Observed molecular weight by Western-blot: 52-55kd,57-66kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARF GAP 3 antibody; ARFGAP1 antibody; ARFGAP3 antibody; FLJ45618 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARFGAP3 recombinant protein (Accession Number: NM_014570). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108252(ARFGAP3 antibody) at dilution of 1:2000
Immunofluorescent analysis of A431 cells using Catalog No:108252(ARFGAP3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108252(ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108252(ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ARFGAP3 represents ADP-ribosylation factor GTPase activating protein 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"