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Product Name
ARFGAP1 Polyclonal Antibody
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Description
Polyclonal antibody to ARFGAP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ARFGAP1 antibody; ARF1GAP antibody; HRIHFB2281 antibody; ADP-ribosylation factor GTPase-activating protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ARFGAP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
Immunohistochemistry - ARFGAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ARFGAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ARFGAP1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using ARFGAP1 antibody . Blue: DAPI for nuclear staining.
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Background
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
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