TPGWamide peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

TPGWamide is a tetrapeptide belonging to the APGWamide-related neuropeptide family, identified in the cephalopod mollusk Sepia officinalis. This peptide, with the sequence Thr-Pro-Gly-Trp-NH₂, is a product of the Mytilus edulis gene and is processed by a dipeptidyl aminopeptidase (DPAP). Tissue mapping via LC-ESI-MS/MS analysis reveals that TPGWamide is exclusively localized within the central nervous system (CNS) of S. officinalis.

In vitro processing studies demonstrate that TPGWamide can be cleaved by DPAP to yield the dipeptide GWamide, although its conversion efficiency is comparatively low. Among related tetrapeptides, TPGWamide shows the lowest processing rate, which is attributed to the influence of its N-terminal threonine residue on DPAP substrate specificity. The presence and specific CNS localization of TPGWamide suggest it is part of a neuropeptide family involved in diverse regulatory functions within molluskan nervous systems.

• Henry J, Zatylny C. Identification and tissue mapping of APGWamide-related peptides in Sepia officinalis using LC-ESI-MS/MS. Peptides. 2002;23(6):1031-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.