-
Product Name
Anti-UBCH8 / UBE2L6 antibody
- Documents
-
Description
Rabbit polyclonal to UBCH8 / UBE2L6
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human UBCH8 / UBE2L6
-
Alternative names
retinoic acid-induced gene B protein antibody; 2810489I21Rik antibody; E2 ubiquitin conjugating enzyme. ubiquitin carrier protein L6 antibody; MGC40331 antibody; RIG-B antibody; RIG-B antibody; RP23-399J8.4 antibody; Ubce8 antibody; UBCH8 antibody; UBCH8 antibody; Ubcm8 antibody; ubiquitin carrier protein L6 antibody; ubiquitin/ISG15-conjugating enzyme E2 L6 antibody; ubiquitin/ISG15-conjugating enzyme E2 L6 antibody; ubiquitin-conjugating enzyme 8 antibody; ubiquitin-protein ligase L6 antibody; ubiquitin-protein ligase L6 antibody; RIG-B antibody; UBCH8 antibody; RIG-B antibody; UBCH8 antibody; Ubce8 antibody; Ubcm8 antibody; 2810489I21Rik antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human UBCH8 / UBE2L6 (rh UBCH8 / UBE2L6; O14933-1; Met1-Ser153). UBCH8 / UBE2L6 specific IgG was purified by Human UBCH8 / UBE2L6 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBCH8 / UBE2L6. The detection limit for Human UBCH8 / UBE2L6 is approximately 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
-
Validations
UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
UBCH8 / UBE2L6 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
-
Background
UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.
-
References
- Moynihan TP, et al. (1999) The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 274(43):30963-8.
- Ardley HC, et al. (2000) Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Cytogenet Cell Genet. 89(1-2):137-40.
- Zhao C, et al. (2004) The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. Proc Natl Acad. 101(20):7578-82.
- Tripathi MK, et al. (2005) Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells. Biochem Biophys Res Commun. 328(1):43-8.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"