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Product Name
Anti-TOP2A antibody
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Description
Rabbit polyclonal to TOP2A
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Tested applications
WB, IHC-P, IF, ICC/IF
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Species reactivity
Human TOP2A
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human TOP2A, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
IHC-P: 0.1-2 μg/mL
ICC/IF: 0.5-1.5 μg/mL
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Validations
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human TOP2A in human ovarian cancer with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human TOP2A in human lung cancer with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human TOP2A in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of TOP2A in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human TOP2A polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"