Anti-TMEM248 antibody

Cat.#: 175131

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Product Information

  • Product Name
    Anti-TMEM248 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to TMEM248
  • Tested applications
    WB, ICC, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    C7orf42 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide corresponding to c-terminal human tmem248.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1,000

    ICC: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.

    Fig1: Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.

    Fig2: ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Flow cytometric analysis of SH-SY-5Y cells with Tmem248 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Fig5: Flow cytometric analysis of SH-SY-5Y cells with Tmem248 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Background

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"