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Product Name
Anti-TMEM248 antibody
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Description
Rabbit polyclonal antibody to TMEM248
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Tested applications
WB, ICC, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
C7orf42 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was synthetic peptide corresponding to c-terminal human tmem248.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1,000
ICC: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of Tmem248 on SkBr3 (1) and K562 cells lysates using anti-Tmem248 antibody.
Fig2: ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Tmem248 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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