Anti-TLE6 antibody

Cat.#: 175120

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Product Information

  • Product Name
    Anti-TLE6 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to TLE6
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Mouse
  • Alternative names
    GRG6 antibody; PREMBL antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within mouse tle6 aa 150-200.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.; Positive control:; Lane 1: Mouse ovary tissue; Lane 2: HepG2

    Fig1: Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution.; Positive control:; Lane 1: Mouse ovary tissue; Lane 2: HepG2

    Fig2: ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.

    Fig6: Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Fig6: Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Background

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"