Anti-TH / Tyrosine Hydroxylase antibody

Anti-TH / Tyrosine Hydroxylase antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Rabbit polyclonal to TH / Tyrosine Hydroxylase

WB, IF, IP, ICC/IF

Human Tyrosine Hydroxylase/TH

DYT14 antibody; OTTMUSP00000020341 antibody; Th antibody; TYH antibody; TYH antibody; DYT14 antibody; DYT5b antibody; Tyrosine Hydroxylase antibody; DYT5b antibody; TH antibody; tyrosine 3-hydroxylase antibody; tyrosine 3-monooxygenase antibody; Tyrosine Hydroxylase antibody

Rabbit IgG

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Tyrosine Hydroxylase/TH, and purified by antigen affinity chromatography.

Polyclonal

0.2 μm filtered solution in PBS

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.

• Daubner SC, et al. (2011) Tyrosine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys. 508(1): 1-12.
• Dunkley PR, et al. (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 91(5): 1025-43.
• Haavik J, et al. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol Neurobiol. 16(3): 285-309.