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Product Name
Anti-TGG2 antibody
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Description
Mouse monoclonal antibody to TGG2
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Tested applications
WB, IHC-P, IF
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Species reactivity
Arabidopsis thaliana
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Alternative names
BETA GLUCOSIDASE 37 antibody; BGLU37 antibody; glucoside glucohydrolase 2 antibody; T1N24.18 antibody; T1N24_18 antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was synthetic peptide within arabidopsis thaliana aa 350-400.
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
IHC-P: 1:200
IF: 1:50-1:200
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Validations
Fig1: Western blot analysis of TGG2 on Arabidopsis thaliana lysates.; Positive control:; Lane 1: Anti-TGG2 antibody, 1/1,000 dilution.; Lane 2: Anti-TGG2 antibody, preincubated with the control peptide antigen.
Fig2: IF staining TGG2 (green) in Arabidopsis thaliana (longitudinal section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: IF staining TGG2 (green) in Arabidopsis thaliana (cross-section). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-TGG2 antibody. Counter stained with hematoxylin.
- Background
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References
- Zhou C et al. Properties of β-thioglucoside hydrolases (TGG1 and TGG2) from leaves of Arabidopsis thaliana. Plant Sci. 191-192:82-92 (2012).
- Islam MM et al. Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells. Plant Cell Physiol 50:1171-1175 (2009).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"