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Product Name
Anti-SHC (1C4) Mouse antibody
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Description
SHC (1C4) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).
Western blot analysis of extracts from A549,MCF7,C6 and Hela cell lysates using Shc mouse mAb (1:1000 diluted).Predicted band size:63KDa.Observed band size:46, 52, 66KDa.
Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher
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Background
Swiss-Prot Acc.P29353.This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
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