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Product Name
Anti-SF3B3 Rabbit antibody
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Description
SF3B3 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
RSE1; SAP130; SF3b130; STAF130 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SF3B3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/50
FC: 1/50
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Validations
Immunocytochemistry of SF3B3(green) in Hela cells using SF3B3 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of SF3B3 in SHSY-5Y,C6 cell lysates using SF3B3 Rabbit pAb(1:1000 diluted).Predicted band size:136KDa.Observed band size:136KDa.
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Background
Swiss-Prot Acc.Q15393.Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
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