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Anti-RAB6B antibody

Cat.#: 175044

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-RAB6B antibody
  • Documents
  • Description
    Mouse monoclonal antibody to RAB6B
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Rat
  • Isotype
    IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of Rab6b on human Rab6b recombinant protein using anti-Rab6b antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of Rab6b on human Rab6b recombinant protein using anti-Rab6b antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of Rab6b on HEK293 (1) and Rab6b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab6b antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of Rab6b on HEK293 (1) and Rab6b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab6b antibody at 1/1,000 dilution.

    Fig3: Western blot analysis of Rab6b on different cell lysate using anti-Rab6b antibody at 1/1,000 dilution.; Positive control:; Lane 1: C6; Lane 2: HT-29; Lane 3: PC-12

    Fig3: Western blot analysis of Rab6b on different cell lysate using anti-Rab6b antibody at 1/1,000 dilution.; Positive control:; Lane 1: C6; Lane 2: HT-29; Lane 3: PC-12

    Fig4: ICC staining Rab6b (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Rab6b (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: ICC staining Rab6b (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: ICC staining Rab6b (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

    Fig8: Flow cytometric analysis of Hela cells with Rab6b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

    Fig8: Flow cytometric analysis of Hela cells with Rab6b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

  • Background
    Q9NRW1
  • References
    • Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014).
    • Petrosyan A & Cheng PW A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi fragmentation by interaction with non-muscle myosin IIA. Glycobiology 23:690-708 (2013).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"