Product Name

Anti-PRIMPOL antibody

Documents

Description

Mouse monoclonal antibody to PRIMPOL

Tested applications

ICC, IHC-P

Species reactivity

Human

Alternative names

MYP22 antibody; CCDC111 antibody; Primpol1 antibody

Isotype

Mouse IgG1

Preparation

This antigen of this antibody was recombinant protein

Clonality

Monoclonal

Formulation

Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Applications

ICC: 1:100-1:200

IHC-P: 1:200

Validations

Fig1: ICC staining CCDC111 in HepG2 cells(red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig2: Immunohistochemical analysis of paraffin-embedded human colonic tissue using anti- CCDC111 mouse monoclonal antibody.

Background

References

hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity. Wan L., Lou J., Xia Y., Su B., Liu T., Cui J., Sun Y., Lou H., Huang J. EMBO Rep. 14:1104-1112(2013)
PrimPol, an archaic primase/polymerase operating in human cells. Garcia-Gomez S., Reyes A., Martinez-Jimenez M.I., Chocron E.S., Mouron S., Terrados G., Powell C., Salido E., Mendez J., Holt I.J., Blanco L. Mol. Cell 52:541-553(2013)
PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication. Bianchi J., Rudd S.G., Jozwiakowski S.K., Bailey L.J., Soura V., Taylor E., Stevanovic I., Green A.J., Stracker T.H., Lindsay H.D., Doherty A.J. Mol. Cell 52:566-573(2013)

Anti-PRIMPOL antibody

Product Information