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Product Name
Anti-PP-X (2F11) Mouse antibody
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Description
PP-X (2F11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Alternative names
PP X;PP-X;PP4;PP4C;PP4C;PP4C_HUMAN;PPH3;PPP4;ppp4c;PPX;protein phosphatase 4 (formerly X) antibody; catalytic subunit;Protein phosphatase 4 catalytic subunit;Protein phosphatase X; Protein phosphatase X;protein phosphatase X antibody; catalytic subunit;Serine/threonine pro antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human Protein Phosphatase 4C protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/200
;ICC: 1/200
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Validations
Immunocytochemistry of HeLa cells using anti-Protein Phosphatase 4C mouse mAb diluted 1:200.
IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb diluted 1/500-1/1000.
Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (1:200 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
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Background
Swiss-Prot Acc.P60510.In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
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