-
Product Name
Anti-Lpar4 antibody
- Documents
-
Description
Rabbit polyclonal antibody to Lpar4
-
Tested applications
WB, IHC-P
-
Species reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep
-
Alternative names
L antibody; Gpr antibody; p2y antibody; LPA4 antibody; p2y9 antibody; Gpr23 antibody; 5730485F04Rik antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human p2y9 175-270/370
-
Clonality
Polyclonal
-
Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
-
Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
-
Applications
WB:1:500-2000
IHC-P:1:400-800
-
Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample: k562 (human)cell Lysate at 40 ug; Primary: Anti- P2Y9 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 42 kD; Observed band size: 48 kD
Fig3: Sample: Brain (mouse) Lysate at 40 ug; Primary: Anti- P2Y9 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 42 kD; Observed band size: 48 kD
Fig4: Sample:; HL-60 Cell (Human) Lysate at 30 ug; Primary: Anti-P2Y9 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD
Fig5: Sample:; Cerebrum (Mouse) Lysate at 40 ug; Ovary (Mouse) Lysate at 40 ug; Primary: Anti-P2Y9 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 42 kD; Observed band size: 57 kD
Fig6: Sample:; HL-60(Human) Cell Lysate at 30 ug; K562(Human) Cell Lysate at 30 ug; Primary: Anti-P2Y9 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 42 kD; Observed band size: 57 kD
- Background
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"