Anti-KAP1 (4E1) Mouse antibody

Cat.#: 168232

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Product Information

  • Product Name
    Anti-KAP1 (4E1) Mouse antibody
  • Documents
  • Description
    KAP1 (4E1) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IHC-P, IP
  • Species reactivity
    Human
  • Alternative names
    E3 SUMO protein ligase TRIM28;E3 SUMO-protein ligase TRIM28;FLJ29029;KAP 1;KAP-1;KRAB associated protein 1;KRAB interacting protein 1;KRAB-associated protein 1;KRAB-interacting protein 1;KRIP 1;KRIP-1;KRIP1;Nuclear corepressor KAP 1;Nuclear corepressor KA antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/100

  • Validations

    IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.

    IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.

    Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.

    Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.

    Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).

  • Background
    Swiss-Prot Acc.Q13263.Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"