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Product Name
Anti-ITGB1 / Integrin beta-1 / CD29 antibody
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Description
Rabbit polyclonal to ITGB1 / Integrin beta-1 / CD29
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Tested applications
WB, IHC-P, IF, IP, ICC/IF
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Species reactivity
Human Integrin beta 1/ITGB1
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Alternative names
CD29 antibody; FNRB antibody; MDF2 antibody; VLAB antibody; GPIIA antibody; MSK12 antibody; VLA-BETA antibody; CD29 antibody; Fnrb antibody; gpIIa antibody; Gm9863 antibody; AA409975 antibody; AA960159 antibody; 4633401G24Rik antibody; ENSMUSG00000051907 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Integrin beta 1/ITGB1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ICC/IF: 0.5-1.5 μg/mL
IP: 4-6 μg/mg of lysate
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Validations
Integrin beta 1 / ITGB1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Integrin beta 1 / ITGB1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ITGB1 in human skin with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Integrin beta 1 / ITGB1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of ITGB1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ITGB1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cell membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"