-
Product Name
Anti-IRF6/IRF-6 antibody
- Documents
-
Description
Mouse monoclonal to IRF6/IRF-6
-
Tested applications
IF, ICC/IF
-
Species reactivity
Human IRF6
-
Alternative names
LPS antibody; PIT antibody; PPS antibody; VWS antibody; OFC6 antibody; PPS1 antibody; mirf6 antibody; AI876454 antibody; E230028I05Rik antibody; VWS1 antibody
-
Isotype
Mouse IgG3
-
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human IRF6. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 10-25 μg/mL
-
Validations
IRF6 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human IRF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IRF6 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"