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Product Name
Anti-Intestinal alkaline phosphatase antibody
- Documents
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Description
Rabbit polyclonal to Intestinal alkaline phosphatase
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Tested applications
ELISA, IHC-P
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Species reactivity
Human ALPI / IAP
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Alternative names
IAP antibody; Akp6 antibody; gIAP antibody; 2010001C14Rik antibody; alkaline phosphomonoesterase antibody; glycerophosphatase antibody; intestinal alkaline phosphatase antibody; intestinal-type alkaline phosphatase antibody; Kasahara isozyme antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ALPI / IAP (rh ALPI / IAP; P09923; Met1-Asp503). ALPI / IAP specific IgG was purified by Human ALPI / IAP affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ALPI / IAP. The detection limit for Human ALPI / IAP is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Alkaline Phosphatase / ALPI Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ALPI in human esophagus with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).Positive staining was localized to epithelium.
Alkaline Phosphatase / ALPI Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ALPI in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).Positive staining was localized to nephric tubule.
Alkaline Phosphatase / ALPI Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ALPI in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).Positive staining was localized to hepatocyte.
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References
- Nakano T, et al. (2009) Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. Am J Physiol Gastrointest Liver Physiol. 297(1): G207-14.
- Henthorn PS, et al. (1987) Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase. Proc Natl Acad Sci. 84(5): 1234-8.
- Oetting WS, et al. (2003) Oculocutaneous albinism type 1: the last 100 years. Pigment Cell Res. 16(3): 307-11.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"