Anti-EEF2/Elongation factor 2 (4B3) Mouse antibody

Cat.#: 168473

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Product Information

  • Product Name
    Anti-EEF2/Elongation factor 2 (4B3) Mouse antibody
  • Documents
  • Description
    EEF2/Elongation factor 2 (4B3) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, ChIP
  • Species reactivity
    Human
  • Alternative names
    EEF2;Eef2;EF-2;EF2;EF2_HUMAN;Elongation factor 2;Eukaryotic translation elongation factor 2;Polypeptidyl tRNA translocase;SCA26. antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant human eEF2 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/5000

    ;ICC: 1/200

  • Validations

    Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.

    Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-eEF2 mouse mAb (dilution 1:200).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-eEF2 mouse mAb (dilution 1:200).

  • Background
    Swiss-Prot Acc.P13639.Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"