Product Name

Anti-Carbonic Anhydrase IV / Car4 / CA4 antibody

Documents

Description

Rabbit polyclonal to Carbonic Anhydrase IV / Car4 / CA4

Tested applications

WB, IHC-P

Species reactivity

Mouse Carbonic Anhydrase IV / Car4 / CA4

Alternative names

Ca4 antibody; CA-IV antibody; Car4 antibody; Carbonic Anhydrase IV antibody; Carbonic Anhydrase IV antibody; CAIV antibody; Car4 antibody; RP17 antibody; RP23-167D6.1 antibody; AW456718 antibody; CA IV antibody

Immunogen

Mouse Carbonic Anhydrase IV / Car4 / CA4 (His Tag) recombinant protein

Isotype

Rabbit IgG

Preparation

Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 (rh Carbonic Anhydrase IV / Car4 / CA4; NP_031633.1; Met1-Ser277). Carbonic Anhydrase IV / Car4 / CA4 specific IgG was purified by Mouse Carbonic Anhydrase IV / Car4 / CA4 affinity chromatography.

Clonality

Polyclonal

Formulation

0.2 μm filtered solution in PBS

Storage instructions

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications

WB: 2-10 μg/ml

IHC-P: 0.1-1 μg/ml

Validations

Carbonic Anhydrase IV / Car4 / CA4 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

Carbonic Anhydrase IV / Car4 / CA4 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO₂ hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3^- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.

Anti-Carbonic Anhydrase IV / Car4 / CA4 antibody

Product Information

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