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Product Name
ANKRD13B antibody
- Documents
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Description
ANKRD13B Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in mouse small intestine tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ANKRD13B antibody; ankyrin repeat domain 13B antibody; FLJ20418 antibody; FLJ25555 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANKRD13B recombinant protein (Accession Number: NM_152345). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:108067(ANKRD13B Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108067(ANKRD13B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108067(ANKRD13B Antibody) at dilution of 1:50 (under 40x lens)
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Background
ANKRD13B (ankyrin repeat domain 13B) is an ubiquitin-binding protein belongs to the Ankrd 13 family. It specifically recognizes and binds 'Lys-63'-linked ubiquitin, and positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (PMID: 22298428). This antibody specifically recognises the 70 kDa protein.
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