-
Product Name
ANKRD13A antibody
- Documents
-
Description
ANKRD13A Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 67 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
ANKRD13 antibody; ANKRD13A antibody; ankyrin repeat domain 13A antibody; NY REN 25 antibody; Protein KE03 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ANKRD13A recombinant protein (Accession Number: NM_033121). Purification method: Antigen Affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108066(ANKRD13A Antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells using Catalog No:108066(ANKRD13A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
ANKRD13A, also named as ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"