Angiotensin I peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Angiotensin I is a decapeptide hormone that serves as a key precursor in the renin-angiotensin system. Its primary sequence is Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu. The conversion of Angiotensin I to the active octapeptide Angiotensin II is a critical regulatory step. This conversion can occur via multiple enzymatic pathways. The classical and well-characterized route is mediated by angiotensin-converting enzyme (ACE).

An alternative, ACE-independent pathway for Angiotensin II generation has also been identified in cardiovascular tissues across several species. Research indicates that chymase, a serine protease stored in mast cell granules, can function as a major Angiotensin II-forming enzyme. Studies in model systems, such as mice, demonstrate that specific chymase isoforms, particularly types 4 and 5, exhibit efficient catalytic activity for this conversion. The activity of this chymase-mediated pathway is localized to the interstitial compartment of blood vessels and the heart, spatially distinct from the luminal ACE activity.

• Li M, Liu K, Michalicek J, Angus JA, Hunt JE, Dell'Italia LJ, Feneley MP, Graham RM, Husain A. Involvement of chymase-mediated angiotensin II generation in blood pressure regulation. J Clin Invest. 2004 Jul;114(1):112-20. doi: 10.1172/JCI20805. PMID: 15232618; PMCID: PMC437969.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.